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OP-012 Gut microbiome in adolescents with obesity
  1. Elizaveta Klimenko,
  2. Natalia Belkova,
  3. Anna Pogodina,
  4. Lyubov Rychkova,
  5. Olga Bugun
  1. Scientific Сentre for Family Health and Human Reproduction Problems


Aim The aim of this study was to characterize the gut microbiome of adolescents with obesity.

Material and Method The study sample consisted of 40 adolescents aged 11–17 years with obesity (n=18) and normal body weight (n=22). Faecal samples obtained from patients were processed according to the standard operating procedures of the International Human Microbiome Standards (IHMS) project, and the V3-V4 amplicons of the 16S rRNA gene variable regions were sequenced. Primary data were deposited in the international database NCBI SRA as BioProject PRJNA604466.

Results Representatives of the phyla Bacteroidetes (46.35±1.88%), Firmicutes (43.17±2.14%), Proteobacteria (7.45±0.83%), and Actinobacteria (1.9±0.0%) dominated the intestinal microbiota. Three types of microbiota composition were observed in our adolescent sample. B-type was characterized by dominance of the genus Bacteroides, P-type was characterized by dominance of the genus Prevotella. The mixed type was characterized by the dominance of two or more taxa in approximately equal proportions. The mixed type was more often identified (χ2=4,98; р < 0,05) in obese adolescents than in adolescents with normal body weight. Inverted results were obtained for the B-type. In gut microbiomes, biosynthetic pathways predominated over degradation, assimilation, and utilization pathways. The largest number of genes has been predicted for the pentose phosphate pathway, gondoic acid biosynthesis, starch degradation, and pyruvate fermentation to isobutanol. The presence of adolescents with varying degrees of metabolic activity of the gut microbiome in the main group showed that even with differences in taxonomic composition, the gut microbiota compensates for the absence of certain microbes, realizing the necessary metabolic functions at the expense of other species.

Conclusions The topic of gut microbiota in obesity in adolescents requires more in-depth research. Understanding how the bacterial community functions can lead to optimal diet selection or even individual treatment.

  • gut microbiome
  • adolescents
  • obesity

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