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OP-013 Gut microbiota and obesity in children: analysis of problems and search for new solutions
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  1. Margarita Ustiuzhanina,
  2. Olga Kovtun,
  3. Danila Zornikov
  1. Federal State Budget Educational Institution of Higher Education «Ural state medical university» of the Ministry of Health of the Russian Federation

Abstract

Aim Evaluate the role of changes in the intestinal microbiota and its metabolites in the development of obesity in children 6–9 years old

Material and Method Information was collected on the microbiota of 96 children 6–9 years old with obesity (SDS BMI more than 2.0) and 60 children with normal body weight (SDS BMI more than -1 and less than 1). Microbiota was assessed using stool PCR. Short-chain fatty acids (SCFA) in feces were determined by gas-liquid chromatography. Assessment of inflammation (CRP) - immunoturbidimetric method. Body composition assessment by bioimpedance. Statistics were performed in the R.

Results In obese children, unlike healthy children, L. lactis, Lactobacillaceae, Prevotella spp., C. perfringens group, Enterobacteriales were more often detected. In the cohort of obese children, higher levels of Bifidobacterium spp, Infant bifidobacteria, L. lactis, B. longum subsp longum, B. adolescentis, Coriobacteria, F. prawusnitzii, Streptococcus spp., Prevotella spp., Enterococcus spp., Erysipelotrichaceae were noted. The number of A. muciniphila in obese children was comparable to healthy children, but the proportion of the species was 2 times less! This trend is characteristic of the Alistipes and Parabacteroides groups [figure 1]. In obese children, the amount of microbial metabolites in feces (SCFA) is reduced and their ratio is changed (C2:C3:C4). The amount of SCFA did not correlate with the amount of adipose tissue in obese children (neither with SDS BMI, nor with% of adipose tissue by bioimpedance). Inflammation and its relationship with the amount of adipose tissue in obese children - a marker of early metabolic changes.

Abstract OP-013 Figure 1

Frequency of detection of groups of microorganisms.The figure characterizes the frequency of detection of groups of microorganisms in children with obesity and with normal body weight, statistical method - 2-sided Fisher’s exact test.

Conclusions It is necessary to clarify the possible causative role of intestinal microbiota disturbances in maintaining subclinical immune inflammation in obese children. Next step must be to study of other mechanisms by which the gut microbiota may contribute to the pathophysiology of obesity (role of SCFAs in homeostasis, regulation of basal metabolism, bile acid metabolism).

  • children
  • microbiota
  • PCR
  • obesity

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