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PP-060 Study of platelet function in children with hemorrhagic syndrome
  1. Liliya Selimzyanova
  1. Pediatrics and Child Health Research Institute, Russian Scientific Center of Surgery named after Academician B. V. Petrovsky, Moscow, Russia


Aim To study the state of platelet hemostasis in children with hemorrhagic syndrome in different nosology’s, based on previously established reference interval (RI) values.

Material and Method We observed 389 patients aged from 4 months to 17 years and 11 months. The median age of treatment was 9.2 years (from 4 months to 17 years and 11 months). At the first stage were examined 230 healthy children for established RI platelets aggregation. On the second stage were examined159 patients with hemorrhagic syndrome (HS), which presented of cutaneous and mucous hemorrhage (in present time and in anamnesis. We used different inductors of aggregation in whole blood using ‘Multiplate’ aggregometer (Verum Diagnostic Roche, France). The methodology of our study was specifically designed to determine the RI of platelets. Also we studied some parameters of the plasma hemostasis (Compact Max, Roche, France).

Results Reference intervals for inducers (ADP, TRAP, ASP-tests) in blood with different stabilizers (hirudin, citrate, lithium-heparin) were established. Significant differences of platelet aggregation were founded only in children under 1 year with TRAP inductor in blood stabilized with hirudin. Thrombocytopathy was founded in children with HS at different diseases. Hypoaggregation was detected more frequently with ADP activator - 41,5% cases among all children (majority of patients with tissue dysplasia and nervous system pathology). Hyperaggregation was detected more frequently with arachidonic acid - 22% among all children (majority of patients with cardiovascular pathology). Abnormalities of plasma haemostasis were founded in 3% of patients with HS. Disturbed in plasma hemostasis was not founded in patients with modified aggregation.

Conclusions The study of our results demonstrates that symptoms of HS may be indicative about the manifestations of thrombocytopathies in most cases, which are important to diagnose in time.

  • aggregation
  • hemorrhagic syndrome
  • reference interval.

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