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Increased levels of Helicobacter pylori IgG antibodies in aqueous humor of patients with primary open-angle and exfoliation glaucoma

  • Clinical Investigation
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Abstract

Purpose

A prospective, nonrandomized, comparative study was carried out to investigate the levels of anti-H. pylori-specific IgG antibodies in the aqueous humor and serum of patients with primary open-angle glaucoma (POAG) and patients with exfoliation glaucoma (XFG), and to compare them with those in age-matched cataract patients.

Methods

Aqueous humor was aspirated at the beginning of glaucoma surgery from 26 eyes of 26 patients with POAG, 27 eyes of 27 patients with XFG, and at the beginning of phakoemulsification cataract surgery from 31 eyes of 31 age-matched normotensive cataract patients. Serum samples were obtained the day before surgery from all patients. Anti-H. pylori IgG concentration in the aqueous humor and serum was measured by means of an enzyme-linked immunosorbent assay.

Results

The mean concentration (± SE) of anti-H. pylori-specific IgG was significantly greater in the aqueous humor samples from patients with POAG (14.27±3.86 U/ml) and XFG (14.25±3.39 U/ml) than in those from age-matched control cataract patients (4.67±1.07 U/ml); (P=0.006 and P=0.003, respectively). No difference was observed in the levels of H. pylori-specific IgG antibodies between POAG and XFG aqueous humor samples (P=0.5). Similarly, the serum concentration of anti-H. pylori antibodies was significantly greater in patients with POAG (69.96±9.69 U/ml; P=0.013) and XFG (81.37±10.62 U/ml; P=0.002) than in the cataract controls (44.16±6.48 U/ml). The mean vertical cupping correlated significantly with the titer of anti-H. pylori-specific IgG in the aqueous humor in the POAG patients.

Conclusion

H. pylori-specific IgG antibody levels are significantly increased in the aqueous humor and serum of patients with POAG and XFG. Moreover, the titer of anti-H. pylori antibody in the aqueous humor might reflect the severity of glaucomatous damage in POAG patients. These findings support a role for H. pylori infection in the pathobiology of these two common glaucomas.

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References

  1. Aragona P, Magazzu G, Macchia G, Bartolone S, Di Pasquale G, Vitali C, Ferreri G (1999) Presence of antibodies against Helicobacter pylori and its heat-shock protein 60 in the serum of patients with Sjögren’s syndrome. J Rheumatol 26:1306–1311

    CAS  PubMed  Google Scholar 

  2. Basso D, Gallo N, Zambon CF, Baron M, Navaglia F, Stockreiter E, Di Mario F, Rugge M, Plebani M (2000) Antigastric autoantibodies in Helicobacter pylori infection: role in gastric mucosal inflammation. Int J Clin Lab Res 30:173–178

    Article  CAS  PubMed  Google Scholar 

  3. Cartwright MJ, Grajewski AL, Friedberg ML, Anderson DR, Richards DW (1992) Immune-related disease and normal-tension glaucoma. A case-control study. Arch Ophthalmol 110:500-502

    CAS  PubMed  Google Scholar 

  4. Chiba S, Sugiyama T, Yonekura K, Tanaka S, Matsumoto H, Fujii N, Ebisu S, Sekiguchi K (2002) An antibody to VacA of Helicobacter pylori in cerebrospinal fluid from patients with Guillain-Barre syndrome. J Neurol Neurosurg Psychiatry 73:76–78

    Article  CAS  PubMed  Google Scholar 

  5. Croinin TO, Clyne M, Appelmelk BJ, Drumm B (2001) Antigastric autoantibodies in ferrets naturally infected with Helicobacter mustelae. Infect Immun 69:2708–2713

    Article  CAS  PubMed  Google Scholar 

  6. Galloway PH, Warner SJ, Morshed MG, Mikelberg FS (2003) Helicobacter pylori infection and the risk for open-angle glaucoma. Ophthalmology 110:922–925

    Article  PubMed  Google Scholar 

  7. Gasbarrini A, Franceschi F, Armuzzi A, Ojetti V, Candelli M, Torre ES, De Lorenzo A, Anti M, Pretolani S, Gasbarrini G (1999) Extradigestive manifestations of Helicobacter pylori gastric infection [review]. Gut 45 [Suppl 1]: I9–I12

  8. Gil-Carrasco F, Vargas-Alarcon G, Zuniga J, Tinajero-Castaneda O, Hernandez-Martinez B, Hernandez-Pacheco G, Rodriguez-Reyna TS, Hesiquio R, Gamboa R, Granados J (1999) HLA-DRB and HLA-DQB loci in the genetic susceptibility to develop glaucoma in Mexicans. Am J Ophthalmol 128:297–300

    Article  PubMed  Google Scholar 

  9. Grau AJ, Buggle F, Lichy C, Brandt T, Becher H, Rudi J (2001) Helicobacter pylori infection as an independent risk factor for cerebral ischemia of atherothrombotic origin. J Neurol Sci 186:1–5

    Article  CAS  PubMed  Google Scholar 

  10. Haefliger IO, Dettmann E, Liu R, Meyer P, Prunte C, Messerli J, Flammer J (1999) Potential role of nitric oxide and endothelin in the pathogenesis of glaucoma. Surv Ophthalmol 43 [Suppl 1]:S51–S58

    Google Scholar 

  11. Harris A, Chung HS, Ciulla TA, Kagemann L (1999) Progress in measurement of ocular blood flow and relevance to our understanding of glaucoma and age-related macular degeneration. Prog Ret Eye Res 18:669–687

    Article  Google Scholar 

  12. Klaver CC, Wolfs RC, Assink JJ, van Duijn CM, Hofman A, de Jong PT (1998) Genetic risk of age-related maculopathy. Population-based familial aggregation study. Arch Ophthalmol 116:1646–1651

    Google Scholar 

  13. Koliakos GG, Konstas AGP, Schrehardt-Schlötzer U, Bufidis T, Georgiadis N, Ringvold A (2002) Ascorbic acid concentration is reduced in the aqueous humor of patients with exfoliation syndrome. Am J Ophthalmol 134:879–883

    Article  PubMed  Google Scholar 

  14. Koliakos GG, Konstas AGP, Schrehardt-Schlötzer U, Hollo G, Katsimbris IE, Georgiadis N, Ritch R (2003) 8-Isoprostaglandin F2a and ascorbic acid concentration in the aqueous humour of patients with exfoliation syndrome. Br J Ophthalmol 87:353–356

    Article  CAS  Google Scholar 

  15. Kountouras J, Mylopoulos N, Boura P, Bessas C, Chatzopoulos D, Venizelos J, Zavos C (2001) Relationship between Helicobacter pylori infection and glaucoma. Ophthalmology 108:599–604

    Article  CAS  PubMed  Google Scholar 

  16. Kountouras J, Mylopoulos N, Chatzopoulos D, Zavos C, Boura P, Konstas AGP, Venizelos J (2002) Eradication of Helicobacter pylori may be beneficial in the management of chronic open-angle glaucoma. Arch Intern Med 162:1237–1244

    Article  PubMed  Google Scholar 

  17. Kornberg AJ, Pestronk A (1993) Immune-mediated neuropathies. Curr Opin Neurol 6:681–687

    CAS  PubMed  Google Scholar 

  18. Mitchell HM, Mascord K, Hazell SL, Daskalopoulos G (2001) Association between the IgG subclass response, inflammation and disease status in Helicobacter pylori infection. Scand J Gastroenterol 36:149–155

    Article  CAS  PubMed  Google Scholar 

  19. Moran AP, Prendergast MM (2001) Molecular mimicry in Campylobacter jejuni and Helicobacter pylori lipopolysaccharides: contribution of gastrointestinal infections to autoimmunity. J Autoimmun 16:241–256

    Article  CAS  PubMed  Google Scholar 

  20. Ozturk F, Kurt E, Inan UU, Cetinkaya Z, Altindis M (2000) Is Helicobacter pylori related to glaucoma? In: Program and abstracts of the 6th Congress of the European Glaucoma Society, London, p 108 [abstract]

  21. Parente F, Negrini R, Imbesi V, Maconi G, Sainaghi M, Vago L, Porro GB (2001) Presence of gastric autoantibodies impairs gastric secretory function in patients with Helicobacter pylori-positive duodenal ulcer. Scand J Gastroenterol 36:474–478

    Article  CAS  PubMed  Google Scholar 

  22. Ringvold A (1999) Epidemiology in the pseudo-exfoliation syndrome. Acta Ophthalmol Scand 77:371–375

    CAS  Google Scholar 

  23. Ritch R (2002) Exfoliation syndrome: more than meets the eye. Acta Ophthalmol Scand 80:465–467

    Article  PubMed  Google Scholar 

  24. Ritch R, Schlötzer-Schrehardt U (2001) Exfoliation syndrome. Surv Ophthalmol 45:265–315

    Article  CAS  PubMed  Google Scholar 

  25. Ritch R, Schlötzer-Schrehardt U, Konstas AG (2003) Why is glaucoma associated with exfoliation syndrome? Prog Ret Eye Res 22:253–275

    Google Scholar 

  26. Ritch R, Shields MB, Krupin T (1996) The glaucomas, 2nd edn. Mosby, St Louis

    Article  Google Scholar 

  27. Romano C, Barrett DA, Li Z, Pestronk A, Wax MB (1998) Anti-rhodopsin antibodies in sera from patients with normal pressure glaucoma. Invest Ophthalmol Vis Sci 116:993–1001

    Google Scholar 

  28. Sheu BS, Shiesh SC, Yang HB, Su IJ, Chen CY, Lin XZ (1997) Implications of Helicobacter pylori serological titer for the histological severity of antral gastritis. Endoscopy 29:27–30

    CAS  PubMed  Google Scholar 

  29. Showji Y, Nozawa R, Sato K, Suzuki H (1996) Seroprevalence of Helicobacter pylori infection in patients with connective tissue diseases. Microbiol Immunol 40:499–503

    CAS  PubMed  Google Scholar 

  30. Singh RK, McMahon AD, Patel H, Packard CJ, Rathbone BJ, Samani NJ (2002) Prospective analysis of the association of infection with CagA bearing strains of Helicobacter pylori and coronary heart disease. Heart 88:43–46

    Article  CAS  PubMed  Google Scholar 

  31. Tezel G, Edward DP, Wax MB (1999) Serum autoantibodies to optic nerve head glycosaminoglycans in patients with glaucoma. Arch Ophthalmol 117:917–924

    PubMed  Google Scholar 

  32. Vorobjova T, Faller G, Maaroos HI, Sipponen P, Villako K, Uibo R, Kirchner T (2000) Significant increase in antigastric autoantibodies in a long-term follow-up study of H. pylori gastritis. Virchows Arch 437:37–45

    Article  CAS  PubMed  Google Scholar 

  33. Wax MB, Tezel G, Saito I, Gupta RS, Harley JB, Li Z, Romano C (1998) Anti-Ro/SS-A positivity and heat shock protein antibodies in patients with normal pressure glaucoma. Am J Ophthalmol 125:145–157

    Article  CAS  PubMed  Google Scholar 

  34. Weisschuh N, Schiefer U (2003) Progress in the genetics of glaucoma. Dev Ophthalmol 37:83–93

    CAS  PubMed  Google Scholar 

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Correspondence to Jannis Kountouras.

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Kountouras, J., Mylopoulos, N., Konstas, A.G.P. et al. Increased levels of Helicobacter pylori IgG antibodies in aqueous humor of patients with primary open-angle and exfoliation glaucoma. Graefe's Arch Clin Exp Ophthalmol 241, 884–890 (2003). https://doi.org/10.1007/s00417-003-0779-5

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  • DOI: https://doi.org/10.1007/s00417-003-0779-5

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