1887

Abstract

The Mycobacteria Growth Indicator Tube (MGIT 960) system was evaluated against Lowenstein–Jensen (LJ) medium and the BACTEC 460 TB system for the recovery of mycobacteria from 1393 consecutive urine specimens. The MGIT had a sensitivity of 91.3 % [95 % confidence interval (CI), 83.2–99.4] when the combination of BACTEC 460 and LJ medium was used as the reference method. The mean time for positivity for MGIT and BACTEC 460 was 19.3 days and 20 days, respectively, while that for LJ medium was 35 days.The incidence of contamination was highest for LJ medium (=148), followed by MGIT 960 (=81), and BACTEC 460 had the lowest incidence of contamination (=50). In conclusion, the isolation of mycobacteria from urine specimens by the MGIT 960 is comparable to that of the BACTEC 460 TB system and solid media.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.2008/001685-0
2008-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/10/1220.html?itemId=/content/journal/jmm/10.1099/jmm.0.2008/001685-0&mimeType=html&fmt=ahah

References

  1. Butler W. R., Floyd M. M., Silcox V., Cage G., Desmond E. other authors 1996 Standardized Method for HPLC Identification of Mycobacteria Atlanta, GA: Centers for Disease Control and Prevention;
    [Google Scholar]
  2. Collins C. H., Grange J. M., Yates M. D. 1997 Tuberculosis Bacteriology: Organisation and Practice , 2nd edn. Butterworth Heinemann London:
    [Google Scholar]
  3. Cruciani M., Scarparo C., Malena M., Bosco O., Serpelloni G., Mengoli C. 2004; Meta-analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without solid media for detection of mycobacteria. J Clin Microbiol 42:2321–2325 [CrossRef]
    [Google Scholar]
  4. Hillemann D., Richter E., Rüsch-Gerdes S. 2006; Use of the BACTEC Mycobacteria Growth Indicator Tube 960 automated system for recovery of mycobacteria from 9,558 extrapulmonary specimens, including urine samples. J Clin Microbiol 44:4014–4017 [CrossRef]
    [Google Scholar]
  5. Master R. N. 1992; Mycobacteriology. In Clinical Microbiology Procedures Handbook Edited by Isenberg H. D. Washington, DC: ASM Press;
    [Google Scholar]
  6. NICE 2006 Tuberculosis: Clinical Diagnosis and Management of Tuberculosis, and Measures for its Prevention and Control London, UK: National Institute for Health and Clinical Excellence;
    [Google Scholar]
  7. Somoskovi A., Kodmon C., Lantos A., Bartfai Z., Tamasi L., Fuzy J., Magyar P. 2000; Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Lowenstein–Jensen medium. J Clin Microbiol 38:2395–2397
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.2008/001685-0
Loading
/content/journal/jmm/10.1099/jmm.0.2008/001685-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error